SUBTYPES OF PROTEIN-KINASE-C IN ISOLATED NERVE GROWTH CONES - ONLY TYPE-II IS ASSOCIATED WITH THE MEMBRANE SKELETON FROM GROWTH CONES

The growth cone particle (GCP) fraction was isolated from fetal and neonatal rat brains and the distribution of protein kinase C subtypes in the fraction was examined by using subtype-specific antibodies. The main subtype in the GCP fraction from fetal forebrain was type II, and type III was also present, but not type I. The pattern was not altered from embryonic day 17 to postnatal day 5. The membrane skeleton subfraction from the GCP fraction contained type II, but far less amount of type III. Our results suggest that type II and type III may be closely related to the functions of growth cones but that they appear to be associated with distinct signal transduction processes. © 1991.