MARKER RESCUE OF A TRANSFORMATION-NEGATIVE EPSTEIN-BARR-VIRUS RECOMBINANT FROM AN INFECTED BURKITT-LYMPHOMA CELL-LINE - A METHOD USEFUL FOR ANALYSIS OF GENES ESSENTIAL FOR TRANSFORMATION

被引：16

作者：

MARCHINI, A

KIEFF, E

LONGNECKER, R

机构：

[1] HARVARD UNIV,SCH MED,DEPT MED,75 FRANCIS ST,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT MOLEC GENET,BOSTON,MA 02115

来源：

JOURNAL OF VIROLOGY | 1993年 / 67卷 / 01期

关键词：

D O I：

10.1128/JVI.67.1.606-609.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Burkitt lymphoma cell line infected in vitro with a transformation-defective mutant recombinant Epstein-Barr virus (EBV) was used to attempt marker rescue of transformation competence by transfection with cloned wild-type DNA. EBV replication was induced in the transfected cells, and wild-type EBV DNA recombined via flanking homologous sequences adjacent to the deletion, resulting in a virus which transformed primary B lymphocytes in vitro. This strategy should be useful for molecular genetic analysis of the role of part or all of any gene in cell growth transformation.

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页码：606 / 609

页数：4

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