CHEMONUCLEOLYSIS WITH CALPAIN-I IN RABBITS

被引：14

作者：

WAKITA, S

SHIMIZU, K

SUZUKI, K

NAKAGAWA, Y

YAMAMOTO, S

YAMAMURO, T

机构：

[1] Department of Orthopaedic Surgery, Faculty of Medicine, Kyoto University, Kyoto

来源：

SPINE | 1993年 / 18卷 / 01期

关键词：

CALPAIN-I; CALPASTATIN; CHEMONUCLEOLYSIS;

D O I：

10.1097/00007632-199301000-00022

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Calpain I is a calcium-dependent cysteine proteinase that has been recently shown to degrade proteoglycan in vitro. The authors injected calpain I, which was purified from human red blood cells, into the intervertebral discs of rabbits. Roentgenograms showed disc space narrowing 1 week after the injection. Histologically, proteoglycan of the nucleus pulposus and anulus fibrosus decreased and notochordal cells in the nucleus pulposus almost disappeared. Biochemical data of the nucleus pulposus showed that the amounts of smaller proteoglycans increased 1 and 4 weeks after the injection. Eight weeks after the injection, histologic and biochemical data showed recovery compared with the data 1 week after injection. These findings show that calpain I is as,potent an enzyme as chondroitinase ABC and has milder chemonucleolytic action than chymopapain. Regarding its possible clinical application, autogenous calpain I as purified from the patient's own red blood cells may have advantages over chymopapain and chondroitinase ABC in that it will prevent anaphylactic reaction.

引用

页码：159 / 164

页数：6

共 19 条

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