ENZYME DEFECTS IN GLUTAMATE-REQUIRING STRAINS OF SCHIZOSACCHAROMYCES-POMBE

被引：9

作者：

BAREL, I ^{[1
]}

MACDONALD, DW ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,DEPT GENET,CAMBRIDGE CB2 3EH,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 03期

关键词：

SCHIZOSACCHAROMYCES POMBE; GLUTAMATE AUXOTROPH; GLUTAMINE-2-OXOGLUTARATE AMINOTRANSFERASE (GOGAT); ISOCITRATE DEHYDROGENASE;

D O I：

10.1016/0378-1097(93)90216-O

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Among the glutamate-requiring strains of Schizosaccharomyces pombe previously described [1], glu2 and glu3 strains were both shown to lack NAD-specific isocitrate dehydrogenase. glu4 strains were shown to lack glutamine:2-oxoglurarate aminotransferase (GOGAT), and to be defective in ammonia assimilation. The regulation of GOGAT activity in wild-type cells was investigated and was consistent with GOGAT and glutamine synthetase being involved in ammonium assimilation, particularly under conditions of nitrogen limitation.

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页码：267 / 272

页数：6

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