FLAVOHAEMOGLOBIN HMPX - A NEW PATHOGENICITY DETERMINANT IN ERWINIA-CHRYSANTHEMI STRAIN-3937

被引:63
作者
FAVEY, S
LABESSE, G
VOUILLE, V
BOCCARA, M
机构
[1] UNIV PARIS 06,DEPT MACROMOLEC BIOL,MINERAL CRISTALLOG LAB,CNRS,URA 09,F-75252 PARIS,FRANCE
[2] UNIV PARIS 07,DEPT MACROMOLEC BIOL,MINERAL CRISTALLOG LAB,CNRS,URA 09,F-75252 PARIS,FRANCE
来源
MICROBIOLOGY-UK | 1995年 / 141卷
关键词
ANAEROBIOSIS; BACTERIAL HEMOGLOBIN; ERWINIA CHRYSANTHEMI; HMP; PECTATE LYASE;
D O I
10.1099/13500872-141-4-863
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Unlike wild-type Erwinia chrysanthemi strain 3937, which fully macerates inoculated Saintpaulia plants, HmpX(-) mutants produce necrotic lesions or no symptoms. The hmpX gene was sequenced and the corresponding protein sequence analysed. We show that HmpX belongs to a family of flavohaemoproteins (HMP), previously identified in two yeasts and in Escherichia coli. Comparisons of protein sequences at the secondary structure level by hydrophobic cluster analysis have shown that HmpX possesses two functional regions, a haemoglobin domain in its N-ternainal part and a flavin reductase domain in its C-terminal part. In an HmpX(-) strain, the synthesis of pectate lyases, which are pathogenicity determinants in E. chrysanthemi, was reduced in conditions of low oxygen tension. Using gus fusion in hmpX, it was shown that hmpX transcription was induced in coculture with tobacco cells. A putative function for HmpX is discussed.
引用
收藏
页码:863 / 871
页数:9
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