CONSTRUCTION AND CHARACTERIZATION OF A BACTERIAL ARTIFICIAL CHROMOSOME LIBRARY OF ARABIDOPSIS-THALIANA

被引：77

作者：

CHOI, S ^{[1
]}

CREELMAN, RA ^{[1
]}

MULLET, JE ^{[1
]}

WING, RA ^{[1
]}

机构：

[1] TEXAS A&M UNIV,CTR CROP BIOTECHNOL,COLLEGE STN,TX 77843

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1995年 / 13卷 / 02期

关键词：

D O I：

10.1007/BF02668782

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed an ordered 3,948-clone arabidopsis BAC Library. The library has a combined average insert size of 100 kb (n = 54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

引用

页码：124 / 128

页数：5

共 5 条

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[4] Woo S.-S., Jiang J., Gill B.S., Paterson A.H., Wing R.A., Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor, Nucleic Acid Res., 22, pp. 4922-4931, (1994)
[5] Zhang H.-B., Zhao Z., Ding X., Paterson A.H., Wing R.A., Preparation of megabase-size DNA from plant nuclei, Plant J, 7, pp. 175-184, (1995)

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