THE GENETIC-CONTROL OF TISSUE-SPECIFIC PEROXIDASES, PER-1, PER-2, PER-3, PER-4, AND PER-5 IN WHEAT

Isoelectric focusing (IEF) of extracts from different tissues of hexaploid wheat cv "Chinese Spring" provided a method of distinguishing and identifying the four known, and one newly discovered, sets of genes encoding peroxidase isozyme production. Per-1, carried on the short arms of homoeologous group 1 chromosomes, shows a high degree of conservation and is active in coleoptile tissue. Per-2, carried on the short arms of group 2 chromosomes, shows some polymorphism and is most active in root tissue. Per-3, on the long arms of group 3 chromosomes, is highly variable and most active in embryo tissue. Per-4, carried on chromosome arms 7AS, 4AL, and 7DS, is quite variable and most active in endosperm tissue. (The chromosome nomenclature used in this paper is that agreed to by the 7th International Wheat Genetics Symposium, where the previous designations of 4A and 4B were reversed.) Restriction fragment length polymorphism (RFLP)-based maps of the group 7 chromosomes were used to locate Per-A4 to a distal region of 7AS. In addition, a further set of genes was identified as being active in root tissue. In wheat a single locus, Per-D5, was found on chromosome arm 2DS. © 1990 Springer-Verlag.