MUSCARINIC RECEPTOR-BINDING IN THE RAT ADRENAL-MEDULLA

Muscarinic receptor binding sites are present in rat adrenal gland and are associated primarily with the medulla. A Scatchard analysis using the ligand [3H]-quinuclidinyl benzylate (3H-QNB) revealed a KD of 0.064 nM and a Bmax of 66 fmol/mg prot. Denervation of the adrenal gland had no significant effect on 3H-QNB binding. The presence of muscarinic receptor binding sites in the adrenal medulla is consistent with reports that muscarinic receptors play a role in the release of adrenal catecholamines and modulation of cyclic 3′,5′-guanosine monophosphate. © 1979.