DETERMINATION OF PLASMA-CATECHOLAMINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - COMPARISON WITH A RADIOENZYMATIC METHOD

被引：647

作者：

HJEMDAHL, P

DALESKOG, M

KAHAN, T

机构：

[1] Department of Pharmacology Karolinska Institutet

来源：

LIFE SCIENCES | 1979年 / 25卷 / 02期

关键词：

D O I：

10.1016/0024-3205(79)90384-9

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

High performance liquid chromatography with electrochemical detection has been compared to a radioenzymatic method for the determination of plasma catecholamines. With the use of an internal standard highly accurate determinations of plasma noradrenaline, adrenaline and dopamine were performed on 0.2-2 ml plasma with the chromatographic method. The radioenzymatic method required only 3 × 50 μl plasma. A comparison of noradrenaline and adrenaline concentrations measured by the two methods in a set of nine plasma samples showed an excellent agreement between the methods (r=0.993 and 0.994, respectively). Advantages and disadvantages with the two methods are discussed. © 1979.

引用

页码：131 / 138

页数：8

共 13 条

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ANTON AH, 1962, J PHARMACOL EXP THER, V138, P360

[2]

CRYER P E, 1976, Diabetes, V25, P1071

[3]

SIMULTANEOUS RADIOENZYMATIC DETERMINATION OF PLASMA AND TISSUE ADRENALINE, NORADRENALINE AND DOPAMINE WITHIN FEMTOMOLE RANGE [J].