PRELIMINARY CHARACTERIZATION OF ANGIOGENIC ACTIVITY IN MEDIA CONDITIONED BY CELLS FROM LUTEINIZED RAT OVARIES

被引：18

作者：

RONE, JD ^{[1
]}

GOODMAN, AL ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL,BALTIMORE,MD 21205

来源：

ENDOCRINOLOGY | 1990年 / 127卷 / 06期

关键词：

D O I：

10.1210/endo-127-6-2821

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Angiogenic activity was detected in media conditioned by ovarian cells from superovulated, pseudopregnant (PMSG/human CG treated) immature Holtzman rats. Media conditioned by cells from luteinized rat ovaries stimulated the directed migration of rabbit endothelial cells or mouse Balb/c3T3 cells, but was not mitogenic to either cell type. That endotheliotropic activity was not associated with a steroid was indicated by the finding that chemoattractant activity was detected in fractions after reversed-phase C18chromatography, which removes more than 95% of steroids present in the media, and that chemoattractant activity was precipitated by ammonium sulfate and by ethanol. Full chemoattractant activity was recovered after boiling (95 C for 30 min), lyophilization, dialysis, Sephadex G-25 desalting columns, and pH changes from 3-10. After Sephadex G-200 chromatography, chemoattractant activity emerged at elution volumes corresponding to 20, 000-30, 000 mol wt. Chemoattractant activity was not retained by Concanavalin A-Sepharose or gelatin-Sepharose, and was only partially retained by heparin-agarose. Chemoattractant activity was also partially retained on both cation and anion exchange columns. Our collective findings indicate the presence of a nonsteroidal, heat-stable, pronase-sensitive factor, nominal mol wt of 20, 000- 30, 000, in media conditioned by cells from luteinized rat ovaries; this factor is chemoattractive but not mitogenic to endothelial cells. Ovarian-derived chemoattractant activity appears to be distinct from fibroblast growth factor because it lacked detectable mitogenic activity, and because fibroblast growth factor was not active in our cell migration bioassay. Because stimulation of endothelial cell migration is a key event during angiogenesis, demonstration of an ovarian endotheliotropic chemoattractant is consistent with our hypothesis that angiogenesis factors play a role in the paracrine regulation of ovarian function. © 1990 by The Endocrine Society.

引用

页码：2821 / 2828

页数：8

共 34 条

[1] Maciag T., Angiogenesis, Progress in Hemostasis and Thrombosis, 7, (1984)
[2] D'amore P.A., Thompson R.W., Mechanisms of Angiogenesis, Annu Rev Physiol, 49, (1987)
[3] Angiogenesis: Mechanisms and Pathobiology, (1987)
[4] Tamura H., Greenwald G.S., Angiogenesis and its hormonal control in the corpus luteum of the pregnant rat, Biol Reprod, 36, (1987)
[5] Jakob W., Jentzsch K.D., Maeuersberger B., Oehme P., Demonstration of angiogenesis activity in the corpus luteum of cattle, Exp Pathol, 13, (1977)
[6] Redmer D.A., Rone J.D., Goodman A.L., Evidence for a nonsteroidal angiotropic factor from the primate corpus luteum
[7] stimulation of endothelial cell migration in vitro, Proc Soc Exp Biol Med, 179, (1985)
[8] Redmer D.A., Grazul A.T., Kirsch J.D., Reynolds C.P., Angiogenic activity of bovine corpora lutea at several stages of luteal development, J Reprod Fertil, 82, (1988)
[9] Gospodarowicz D., Cheng J., Lui G.M., Baird A., Esch F., Bohlen P., Corpus luteum angiogenic factor is related to fibroblast growth factor, Endocrinology, 117, (1985)
[10] Neufeld G., Ferrara N., Schweigerer L., Mitchell R., Gospodarowicz D., Bovine granulosa cells produce basic fibroblast growth factor, Endocrinology, 121, (1987)

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