THEOPHYLLINE ESTIMATION - COMPARATIVE EVALUATION OF A GAS-CHROMATOGRAPHIC METHOD AND A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD

被引:6
作者
NAISH, PJ
CHAMBERS, RE
COOKE, M
机构
[1] ROYAL INFIRM,DEPT CHEM PATHOL,BRISTOL BS2 8HW,ENGLAND
[2] UNIV BRISTOL,DEPT INORGAN CHEM,BRISTOL BS8 1TS,AVON,ENGLAND
关键词
D O I
10.1177/000456327901600165
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A comparative evaluation of a gas-liquid chromatographic (GLC) assay and a high-performance liquid chromatographic (HPLC) assay for the determination of plasma theophylline levels has been carried out. Both methods are reliable, accurate, and technically simple and appear to be specific for theophylline. The HPLC system is more sensitive (sample volume 25 μl) than the GLC system (sample volume 500 μl) and is therefore suitable for the estimation of theophylline in microsamples. The HPLC system also has better precision (coefficient of variation: within-batch 2.0%, between-batch 3.0%) than the GLC system (coefficient of variation: within-batch 3.5%, between-batch 5.6%). Good correlation (r=0.964) was found between plasma theophylline levels (n=75, observed range 4-186 μmol/l) determined by each procedure. The results of this study indicate that, of the two systems investigated, HPLC is the method of choice.
引用
收藏
页码:254 / 258
页数:5
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