A NEW METHOD FOR THE PURIFICATION OF THE INFLUENZA A-VIRUS NEURAMINIDASE

被引：46

作者：

MCKIMMBRESCHKIN, JL

CALDWELL, JB

GUTHRIE, RE

KORTT, AA

机构：

[1] CSIRO Division of Biomolecular Engineering, Parkville

来源：

JOURNAL OF VIROLOGICAL METHODS | 1991年 / 32卷 / 01期

关键词：

INFLUENZA; NEURAMINIDASE; PURIFICATION;

D O I：

10.1016/0166-0934(91)90192-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid new method for the purification of neuraminidase (NA) heads from influenza A virus is described. Virus was pelleted directly from allantoic fluid and was digested with pronase. The cores were removed by centrifugation, redigested and the released NA heads were pooled and concentrated. The NA was separated from all contaminating proteins in a single step on a Superose 12 column. The purified material was suitable for both crystallography and for the production of monospecific antisera.

引用

页码：121 / 124

页数：4

共 5 条

[1]

ANSGORGE W, 1982, ELECTROPHORESIS, V3, P235

[2] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].