CHARACTERIZATION OF TRYPSINOGEN-1 AND TRYPSINOGEN-2 IN 2 HUMAN PANCREATIC ADENOCARCINOMA CELL-LINES - CFPAC-1 AND CAPAN-1

被引：25

作者：

MISZCZUKJAMSKA, B

MERTEN, M

GUYCROTTE, O

AMOURIC, M

CLEMENTE, F

SCHOUMACHER, RA

FIGARELLA, C

机构：

[1] GRP RECH GLANDES EXOCRINES, 27 BLVD LEI ROURE, F-13009 MARSEILLE, FRANCE

[2] CHU RANGUEIL, INSERM, U151, F-31052 TOULOUSE, FRANCE

[3] UNIV ALABAMA, DEPT PEDIAT, BIRMINGHAM, AL USA

来源：

FEBS LETTERS | 1991年 / 294卷 / 03期

关键词：

ADENOCARCINOMA CELL LINE; PANCREATIC; TRYPSINOGEN;

D O I：

10.1016/0014-5793(91)80662-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins with trypsin-like immunoreactivity (first detected by a specific immunoenzymatic assay) were isolated from CAPAN-1 and CFPAC-1 cell culture-conditioned media by chromatography on an immunoadsorbent prepared with a polyclonal antibody directed against trypsin 1. The adsorbed proteins were devoid of free trypsin activity but trypsin activity was present after enterokinase activation demonstrating that the immunoreactive trypsin present in cell supernatants corresponds to trypsinogens. When characterised by Western blotting using a monoclonal antibody directed against human trypsin 1 two protein bands corresponding to trypsinogen 1 (23 kDa) and trypsinogen 2 (25 kDa) gave a positive reaction. These results demonstrate the presence of trypsinogens 1 and 2 in CAPAN-1 and CFPAC-1 cells and in their culture-conditioned media.

引用

页码：175 / 178

页数：4

共 28 条

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