ONLINE DETERMINATION OF INTRACELLULAR BETA-GALACTOSIDASE ACTIVITY IN RECOMBINANT ESCHERICHIA-COLI USING FLOW-INJECTION ANALYSIS (FIA)

被引：16

作者：

KRACKEHELM, HA ^{[1
]}

BRANDES, L ^{[1
]}

HITZMANN, B ^{[1
]}

RINAS, U ^{[1
]}

SCHUGERL, K ^{[1
]}

机构：

[1] UNIV HANOVER,INST TECH CHEM,CALLINSTR 3,W-3000 HANNOVER 1,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1991年 / 20卷 / 01期

关键词：

FLOW INJECTION ANALYSIS; FIA; PROCESS MONITORING; INTRACELLULAR ENZYME; BETA-GALACTOSIDASE ACTIVITY;

D O I：

10.1016/0168-1656(91)90038-W

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A flow injection analysis (FIA) system was developed for the determination of cytoplasmic beta-galactosidase activity in recombinant Escherichia coli. The FIA system and its application for on-line monitoring of beta-galactosidase production during cultivation of recombinant E. coli in a 60-1 airlift tower loop reactor is described. The results demonstrate that an FIA assay in conjunction with a cell disintegration step can be applied successfully for on-line monitoring of intracellular protein formation.

引用

页码：95 / 104

页数：10

共 20 条

[1] CONTINUOUS ONLINE MONITORING OF INTRACELLULAR ENZYME-ACTIVITY [J].

AHLMANN, N ;

NIEHOFF, A ;

RINAS, U ;

SCHEPER, T ;

SCHUGERL, K .

ANALYTICA CHIMICA ACTA, 1986, 190 (01) :221-226

[2]

BUHLER H, 1985, MESSEN BIOTECHNOLOGI

[3] ISOLATION AND CHARACTERIZATION OF DELETIONS IN BACTERIOPHAGE-LAMBDA RESIDING AS PROPHAGE IN ESCHERICHIA-COLI K12 [J].

CASTELLAZZI, M ;

BRACHET, P ;

EISEN, H .

MOLECULAR AND GENERAL GENETICS, 1972, 117 (03) :211-+

[4]

FRUH K, 1986, BIOTECH FORUM, V3, P204

[5] CONTINUOUS ASSAY FOR AN INTRACELLULAR ENZYME - ANALYSIS OF ACETATE KINASE IN ESCHERICHIA-COLI [J].

KOPLOVE, HM ;

COONEY, CL .

ANALYTICAL BIOCHEMISTRY, 1976, 72 (1-2) :297-304

[6] FLOW-INJECTION ANALYSIS AND BIOSENSORS - APPLICATIONS FOR BIOTECHNOLOGY AND ENVIRONMENTAL-CONTROL [J].

LUDI, H ;

GARN, MB ;

BATAILLARD, P ;

WIDMER, HM .

JOURNAL OF BIOTECHNOLOGY, 1990, 14 (01) :71-79

[7]

Miller J. H., 1982, EXPT MOL GENETICS

[8] PREREQUISITES FOR THE ONLINE CONTROL OF MICROBIAL PROCESSES BY FLOW-INJECTION ANALYSIS [J].

OGBOMO, I ;

PRINZING, U ;

SCHMIDT, HL .

JOURNAL OF BIOTECHNOLOGY, 1990, 14 (01) :63-70

[9] ONLINE MONITORING OF ENZYMES IN DOWNSTREAM PROCESSING BY FLOW-INJECTION ANALYSIS (FIA) [J].

RECKTENWALD, A ;

KRONER, KH ;

KULA, MR .

ENZYME AND MICROBIAL TECHNOLOGY, 1985, 7 (12) :607-612

[10] RAPID ONLINE PROTEIN-DETECTION IN BIOTECHNOLOGICAL PROCESSES BY FLOW-INJECTION ANALYSIS (FIA) [J].

RECKTENWALD, A ;

KRONER, KH ;

KULA, MR .

ENZYME AND MICROBIAL TECHNOLOGY, 1985, 7 (04) :146-149

← 1 2 →