DIRECT INTERACTION BETWEEN 2 ESCHERICHIA-COLI TRANSCRIPTION ANTITERMINATION FACTORS, NUSB AND RIBOSOMAL-PROTEIN S10

被引：60

作者：

MASON, SW

LI, J

GREENBLATT, J

机构：

[1] UNIV TORONTO,DEPT MOLEC & MED GENET,TORONTO M5S 1A1,ONTARIO,CANADA

[2] UNIV TORONTO,BANTING & BEST DEPT MED RES,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 223卷 / 01期

关键词：

ANTITERMINATION; N PROTEIN; NUSB; S10; PROTEIN-PROTEIN INTERACTIONS;

D O I：

10.1016/0022-2836(92)90715-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli proteins NusB and ribosomal protein S10 are important for transcription antitermination by the bacteriophage lambda N protein. We have used sucrose gradient co-sedimentation and affinity chromatography with immobilized ribosomal protein S10, a glutathione S-transferase-S10 fusion protein, and NusB to show that NusB binds directly and very selectively to S10. The interaction is non-ionic and has an estimated Kd value of 10-7 m. We hypothesize that NusB binds to N-modified transcription complexes primarily by interacting with S10. © 1992.

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页码：55 / 66

页数：12

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