A CORRELATION OF THE OUTCOME OF CLINICAL INVITRO FERTILIZATION WITH THE INOSITOL CONTENT AND EMBRYOTROPHIC PROPERTIES OF HUMAN SERUM

被引：43

作者：

CHIU, TTY

TAM, PPL

机构：

[1] IVF Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T.

[2] Embryology Unit, The Children's Medical Research Institute, University of Sydney, Wentworthville, 2145, NSW

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 06期

关键词：

INOSITOL; HUMAN SERA; EMBRYOTROPHIC PROPERTY; INVITRO FERTILIZATION; POSTIMPLANTATION MOUSE EMBRYO CULTURE;

D O I：

10.1007/BF01204248

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: This study was designed to investigate whether the level of myo-inositol (MI) in human serum is critical for embryotrophic activity. Methods: The embryotrophic properties of human serum were evaluated by the development of postimplantation mouse embryos [in vitro assay of Tam et al. (Fertil Steril 48:834-839, 1987)]. An enzymatic spectrophotometric method using myo-inositol dehydrogenase was used for determination of serum MI. The level of MI detected in serum was compared with the embryotrophic properties and the pregnancy outcome. The effect of MI on the embryotrophic activity of human serum was studied by supplementing the suboptimal serum samples that were unsupportive of embryo growth with extra MI. Results: Serum obtained from patients having successful IVF pregnancies generally supported better development of postimplantation mouse embryos and contained higher levels of inositol, particularly if the serum sample was collected during the IVF treatment cycle. Serum samples obtained from patients with aborted pregnancies, though supporting mouse embryo development, contained significantly lower concentrations of inositol. An improvement of the embryotrophic properties with exogenous inositol supplement was achieved in some but not all of the suboptimal serum samples studied. Conclusions: It is possible that other factors in addition to inositol are crucial in promoting better embryonic development.

引用

页码：524 / 530

页数：7

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