HUMAN IGE MEDIATES STIMULUS-SECRETION COUPLING IN RAT BASOPHILIC LEUKEMIA-CELLS TRANSFECTED WITH THE ALPHA-CHAIN OF THE HUMAN HIGH-AFFINITY RECEPTOR

被引：36

作者：

WILSON, APM ^{[1
]}

PULLAR, CE ^{[1
]}

CAMP, AM ^{[1
]}

HELM, BA ^{[1
]}

机构：

[1] UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOLEC BIOL & BIOTECHNOL,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1993年 / 23卷 / 01期

关键词：

HUMAN IGE; FC-EPSILON-RI; RAT BASOPHILIC LEUKEMIA CELL LINE;

D O I：

10.1002/eji.1830230137

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cell surface expression of the alpha chain of the human (h) Fcepsilon receptor type 1 (FcepsilonR1) can be observed following stable transfection of RBL-2H3 cells with the halpha chain of the FcepsilonR1 complex. Association and dissociation constants for hIgE are similar to those previously reported for the ligand and its receptor. The demonstration of mediator release following the cross-linking of hFcepsilonR1alpha by hIgE and antigen or anti-hFcepsilonR1alpha antibody suggests that this system will assist the identification of structural motifs and specific amino acids that are involved in the generation of the signal(s) that will initiate and/or propagate the secretion of mediators from mast cells and basophils.

引用

页码：240 / 244

页数：5

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