RAPID ACID-HYDROLYSIS OF ALBUMIN IN A MICROWAVE-OVEN

被引：14

作者：

MORALESRUBIO, A ^{[1
]}

CEREZO, J ^{[1
]}

SALVADOR, A ^{[1
]}

DELAGUARDIA, M ^{[1
]}

机构：

[1] UNIV VALENCIA,DEPT ANALYT CHEM,50 DOCTOR MOLINER ST,E-46100 BURJASSOT,SPAIN

来源：

MICROCHEMICAL JOURNAL | 1993年 / 47卷 / 03期

关键词：

D O I：

10.1006/mchj.1993.1043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microwave-oven-assisted acid hydrolysis of albumin in 7.5 M HCl was carried out in only 12 min in a pressurized Teflon reactor using a power of 390 W. The effect of experimental conditions on the hydrolysis of albumin were evaluated and the results obtained were compared with those found by conventional hydrolysis, using in both cases the ninhydrin reaction for the spectrophotometric determination of free amino acids. A chromatographic study showed that microwave oven heating did not change the nature of the amino acids obtained from the hydrolysis of the protein. © 1993 Academic Press. All rights reserved.

引用

页码：270 / 277

页数：8

共 25 条

[1] WET ASHING OF SOME BIOLOGICAL SAMPLES IN A MICROWAVE OVEN [J].

ABUSAMRA, A ;

MORRIS, JS ;

KOIRTYOHANN, SR .

ANALYTICAL CHEMISTRY, 1975, 47 (08) :1475-1477

[2] MODIFICATION OF A MICROWAVE-OVEN FOR LABORATORY USE [J].

ANDREWS, J ;

ATKINSON, GF .

JOURNAL OF CHEMICAL EDUCATION, 1984, 61 (02) :177-178

[3]

CHEN ST, 1987, INT J PEPT PROT RES, V30, P572

[4] RAPID PROTEIN HYDROLYSIS BY MICROWAVE IRRADIATION USING HEAT-RESISTANT TEFLON-PYREX TUBES [J].

CHIOU, SH .

JOURNAL OF THE CHINESE CHEMICAL SOCIETY, 1989, 36 (05) :435-442

[5] PEPTIDE AND PROTEIN HYDROLYSIS BY MICROWAVE IRRADIATION [J].

CHIOU, SH ;

WANG, KT .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 491 (02) :424-431

[6]

CREAMER LK, 1976, J DAIRY SCI TECHNOL, V11, P211

[7] RAPID HYDROLYSIS OF BENZODIAZEPINES TO BENZOPHENONES IN A MICROWAVE-OVEN [J].

DELAGUARDIA, M ;

SALVADOR, A ;

GOMEZ, MJ ;

DEBENZO, ZA .

ANALYTICA CHIMICA ACTA, 1989, 224 (01) :123-126

[8]

GEYDE RN, 1988, CAN J CHEM, V66, P17

[9] APPLICATION OF COMMERCIAL MICROWAVE-OVENS TO ORGANIC-SYNTHESIS [J].

GIGUERE, RJ ;

BRAY, TL ;

DUNCAN, SM ;

MAJETICH, G .

TETRAHEDRON LETTERS, 1986, 27 (41) :4945-4948

[10]

HILL RL, 1962, J BIOL CHEM, V237, P389

← 1 2 3 →