INFLUX AND EFFLUX OF ORGANIC-ACIDS ACROSS THE SOIL-ROOT INTERFACE OF ZEA-MAYS L AND ITS IMPLICATIONS IN RHIZOSPHERE C FLOW

The influx and efflux of organic acids across the root-soil interface were investigated in intact, sterile maize (Zea mays L.) roots under a variety of experimental conditions. Under nutrient-sufficient conditions the efflux of organic acids was shown to constitute < 1% of the total C lost across the root-soil interface. Under severe nutrient stress, however, the rates of malate and citrate efflux from the root increased 33 and 12 fold respectively. Influx experiments indicated that roots could not directly reabsorb citrate-Fe3+ or other metal complexes from solution. Influx of citrate was observed only at high external citrate concentrations (greater than or equal to 1 mM) or from solutions with low ionic strengths. It was postulated that citrate influx is of little importance in a soil environment.