PLASMA VIREMIA TITRATION AND RNA QUANTITATION IN ICD-P24 NEGATIVE HIV TYPE-1-INFECTED PATIENTS

被引:9
作者
ERCOLI, L
SARMATI, L
ELSAWAF, G
COCHI, S
LANTI, T
IUDICONE, P
GUGLIELMETTI, M
GIANNINI, G
GALLUZZO, C
TOMINO, C
ANDREONI, M
VELLA, S
机构
[1] IST SUPER SANITA,VIROL LAB,I-00161 ROME,ITALY
[2] UNIV ROMA TOR VERGATA,DEPT PUBL HLTH & CELLULAR BIOL,ROME,ITALY
[3] CNTS CROCE ROSSA ITALIANA,ROME,ITALY
关键词
D O I
10.1089/aid.1995.11.1203
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Quantitative culture of human immunodeficiency virus (HIV) was performed on 202 plasma samples obtained from asymptomatic and early symptomatic HIV-1 infected patients (mean CD4(+) count: 186/mm(3)) before antiretroviral therapy was started. HIV could be isolated from 84% of the plasma samples (titers ranging from 10(0) to 10(2.75) TCID50/ml). Immune complex dissociated p24 antigen (ICD-p24) was detected in 66% of the samples. Only 23 samples (11%) were negative for both ICD-p24 as well as HIV culture. Discordant results were obtained in 55 samples, and 45 samples negative for ICD-p24 were positive for HIV culture. A significant proportion (42%) of patients that were negative for ICD-p24 belonged to a very advanced group with very low CD4(+) cell count. However, almost 90% of these ICD-p24 negative samples were positive for HIV plasma viremia, stressing the value of this virological marker in patients with low CD4(+) cell count and without any detectable ICD-p24 antigenemia. HIV-1 RNA was detected in all ICD-p24 negative plasma samples tested by the branched DNA (bDNA) assay. A very good correlation was found between high RNA copy number and HIV plasma isolation in samples obtained from patients with low CD4(+) cell count, suggesting that HIV-1 RNA quantitation may also reflect viral infectivity of plasma.
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收藏
页码:1203 / 1207
页数:5
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