GEL-FILTRATION OF EGG PHOSPHATIDYLCHOLINE VESICLES

被引：11

作者：

CHEN, CY

SCHULLERY, SE

机构：

[1] Department of Chemistry, Eastern Michigan University, Ypsilanti

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 03期

关键词：

gel filtration; ohsphatidylcholine; sonication; vesicles;

D O I：

10.1016/0165-022X(79)90037-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The abilities of Sepharose 2b (Pharmacia), Controlled Pore Glass (Electro-Nucleonics) and Bio-Gel A150m (Bio-Rad) to purify small unilamellar vesicles prepared by sonication and the ethanol-injection methods were compared. The Bio-Gel causes complete aggregation of the sonicated vesicles and partial aggregation of the ethanol-injection vesicles. Both Sepharose and Controlled Pore Glass are acceptable for purifying vesicles from multilamellar liposomes; however, neither will separate the vesicles from sonication by-products which might be formed. © 1979.

引用

页码：189 / 192

页数：4

共 21 条

[1] TURBIDITY CHANGES OF LIPID VESICLES NEAR PHASE-TRANSITION TEMPERATURE AS AN INDICATION OF FUSION
AVRAMOVICZIKIC, O
COLBOW, K
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 512 (01) : 97 - 104
[2] SIMPLE METHOD FOR PREPARATION OF HOMOGENEOUS PHOSPHOLIPID VESICLES
BARENHOLZ, Y
GIBBES, D
LITMAN, BJ
GOLL, J
THOMPSON, TE
CARLSON, FD
[J]. BIOCHEMISTRY, 1977, 16 (12) : 2806 - 2810
[3] SINGLE BILAYER LIPOSOMES PREPARED WITHOUT SONICATION
BATZRI, S
KORN, ED
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 298 (04) : 1015 - 1019
[4] MEMBRANE-FUSION - STUDIES WITH A CALCIUM-SENSITIVE DYE, ARSENAZOIII, IN LIPOSOMES
DUNHAM, P
BABIARZ, P
ISRAEL, A
ZERIAL, A
WEISSMANN, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) : 1580 - 1584
[5] MECHANISM OF SONICATION OF AQUEOUS EGG-YOLK LECITHIN DISPERSIONS AND NATURE OF RESULTANT PARTICLES
FINER, EG
FLOOK, AG
HAUSER, H
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 260 (01) : 49 - &
[6] EFFECT OF ULTRASONIC IRRADIATION ON CHEMICAL STRUCTURE OF EGG LECITHIN
HAUSER, HO
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 45 (04) : 1049 - +
[7] STUDIES ON STATE OF PHOSPHATIDYLCHOLINE MOLECULES BEFORE AND AFTER ULTRASONIC AND GEL-FILTRATION TREATMENTS
HUANG, C
CHARLTON, JP
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 46 (04) : 1660 - +
[8] STUDIES ON PHOSPHATIDYLCHOLINE VESICLES . FORMATION AND PHYSICAL CHARACTERISTICS
HUANG, CH
[J]. BIOCHEMISTRY, 1969, 8 (01) : 344 - +
[9] KLEIN RA, 1970, BIOCHIM BIOPHYS ACTA, V210, P486, DOI 10.1016/0005-2736(70)90231-2
[10] MEMBRANE MOBILITY AGENTS .4. MECHANISM OF PARTICLE-CELL AND CELL-CELL FUSION
KOSOWER, EM
KOSOWER, NS
WEGMAN, P
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 471 (02) : 311 - 329

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