EXISTENCE AND SEPARATION OF THE FOLLICLE-STIMULATING HORMONE-RELEASING HORMONE FROM THE LUTEINIZING-HORMONE RELEASING HORMONE

被引：12

作者：

FUCHS, S ^{[1
]}

LUNDANES, E ^{[1
]}

LEBAN, J ^{[1
]}

FOLKERS, K ^{[1
]}

BOWERS, C ^{[1
]}

机构：

[1] TULANE UNIV,SCH MED,NEW ORLEANS,LA 70112

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 88卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91700-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine hypothalamic fragments were extracted by 2M AcOH at 4°C, and the extractives were subsequently processed in the presence of one protease inhibitor and one anti-oxidant. Gel filtration was performed on Bio-Gel P-2, and supplementary [3H]-LHRH and [14C]- <GluOH were used. The fractions of elution were assayed for both LH- and FSH-releasing activities and for radioactivities. An entity which unambiguously released FSH was separated from [3H]-LHRH, and was differentiated from [14C]- <GluOH and N-Ac-AspOH. This entity may be presumed to be FSHRH, and if so, it may have a molecular weight larger than that of the decapeptide, LHRH. © 1979.

引用

页码：92 / 96

页数：5

共 11 条

[1]

BOWERS CY, 1973, BBRC, V50, P14

[2]

FAWCETT CP, 1973, ENDOCRINOLOGY, V96, P1311

[3]

FOLKERS K, 1978, NOVEL ASPECTS REPROD, P319

[4]

FUCHS S, BBRC