LOCALIZATION OF ENDOGENOUS OSTEOCALCIN IN NEONATAL RAT BONE AND ITS ABSENCE IN ARTICULAR-CARTILAGE - EFFECT OF WARFARIN TREATMENT

被引：61

作者：

BOIVIN, G

MOREL, G

LIAN, JB

ANTHOINETERRIER, C

DUBOIS, PM

MEUNIER, PJ

机构：

[1] FAC LYON SUD,CNRS,URA 559,OULLINS,FRANCE

[2] HARVARD UNIV,SCH MED,STUDY SKELETAL DISORDERS LAB,BOSTON,MA 02115

[3] UNIV MASSACHUSETTS,MED CTR,DEPT CELL BIOL,WORCESTER,MA 01605

来源：

VIRCHOWS ARCHIV A-PATHOLOGICAL ANATOMY AND HISTOPATHOLOGY | 1990年 / 417卷 / 06期

关键词：

OSTEOCALCIN; OSTEOBLAST; OSTEOCYTE; ULTRATHIN FROZEN SECTIONS; WARFARIN TREATMENT;

D O I：

10.1007/BF01625731

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Immunocytochemistry after cryoultramicrotomy was used to localize endogenous osteocalcin in bone (calvaria, femoral diaphysis) and epiphyseal femoral cartilage from 8-day-old rats treated (or mot) for 7 days with warfarin. Ultrathin frozen sections were incubated with goat antiserum against rat osteocalcin at high dilutions (2 x 10(-4) to 2 x 10(-6)). In calvaria and femur of untreated rats, endogenous osteocalcin was observed in osteoblasts (cytoplasm and nucleus) and in the collagenous matrix. Osteocalcin appeared progressively in osteoblasts and bone matrix in the mineralization front, then increased in the regions of extended calcification. Osteocalcin was also detected in osteocytes but was not as abundant as in osteoblasts. In bone samples of warfarin-treated rats, endogenous osteocalcin was only detected in bone matrix but not in osteoblasts. Furthermore, osteocalcin was only observed if antiserum was not very dilute (2 x 10(-2)). In cartilage (hypertrophied and degenerative zones), osteocalcin was not observed in matrix and chondrocytes. However, it was found in the vicinity of matrix vesicles at the initial loci of calcification. Osteocalcin was never detected in the cartilage of warfarin-treated rats. Our results provide ultrastructural immunocytological evidence for the localization of endogenous osteocalcin in osteoblasts, the presence of osteocalcin in bone matrix and a direct gradient between the presence of osteocalcin and the calcification process. Osteocalcin is absent from cartilage, except possibly close to calcifying matrix vesicles. Warfarin inhibits the formation of osteocalcin.

引用

页码：505 / 512

页数：8

共 41 条

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