ISFET-BASED UREA BIOSENSOR

被引:14
作者
ALEGRET, S
BARTROLI, J
JIMENEZ, C
MARTINEZFABREGAS, E
MARTORELL, D
VALDESPEREZGASGA, F
机构
[1] Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona
关键词
D O I
10.1016/0925-4005(93)85227-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new construction method of a PVC-matrix membrane ISFET-based urea sensor is described and compared with a pH ISFET-based urea sensor which uses a classical immobilization procedure on a nylon net. The response slope obtained with data from the pH ISFET-urea sensor is 57 mV/decade over a linear working range of 10(-4)-3 x 10(-3) M urea with a lifetime over 20 days. This sensor, applied in a flow-injection system, presents a sensitivity of 60 mV/decade and shows an acceptable sampling rate of 35 samples/h. Although its lifetime is shorter under dynamic conditions the membrane is easily changeable in the flow system. The new sensor reported is based on an ammonium ISFET with the PVC-matrix membrane cast on a graphite/epoxy composite. The enzyme is deposited and covalently fixed onto the PVC membrane by means of a fine spraying of glutaraldehyde. This sensor shows a sensitivity of 42 mV/decade over a linear working range between 4 x 10(-5) and 10(-2) M urea and a long-term stability of one week.
引用
收藏
页码:453 / 457
页数:5
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