IMPAIRED REGULATION OF SURFACTANT PHOSPHOLIPID-METABOLISM IN THE ISOLATED RAT LUNG AFTER NITROGEN-DIOXIDE INHALATION

被引:5
作者
MENGEL, RG
BERNHARD, W
BARTH, P
VONWICHERT, P
MULLER, B
机构
[1] UNIV MARBURG,DEPT INTERNAL MED POLICLIN,PULM RES UNIT,POB 2360,W-3550 MARBURG,GERMANY
[2] UNIV MARBURG,DEPT PATHOL,W-3550 MARBURG,GERMANY
[3] HANNOVER MED SCH,DEPT PHARMACOL & TOXICOL,W-3000 HANNOVER 61,GERMANY
关键词
D O I
10.1006/taap.1993.1105
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Various drugs have been shown to stimulate surfactant phospholipid metabolism. Particularly β-adrenergic agonists play an important role under physiologic conditions. For the first time we have studied whether nitrogen dioxide (NO2) inhalation alters β-adrenergic regulation of surfactant phospholipid metabolism in the model of the isolated lung. Rats were continuously exposed in vivo to a 5 ppm NO2-containing atmosphere for 48 hr. The lungs were isolated and perfused in presence of the β-adrenergic agonist dopexamine and surfactant metabolism was studied in three lung compartments: (1) lung lavage, (2) lung tissue, and (3) lavagable free alveolar cells. We found that (1) in normal rat lungs dopexamine increased the incorporation of palmitate and choline from the perfusate into lung lavage phospholipids. In nitrogen dioxide exposed rat lungs β-adrenergic stimulation did not cause an increase in precursor incorporation. No significant difference in unstimulated precursor incorporation was found for normal and NO2-exposed rat lungs. (2) Lung tissue from rats exposed to NO2 showed a decreased precursor incorporation into disaturated phosphatidylcholine due to an augmented cellular pool size. (3) Lavagable alveolar cells showed an increased palmitate uptake after nitrogen dioxide inhalation and by β- adrenergic stimulation. From these data we conclude that nitrogen dioxide inhalation impairs the β-adrenergic regulation of surfactant phospholipid metabolism. Moreover these data underline the importance of β-adrenergic agonists in surfactant metabolism. © 1994 Academic Press. All rights reserved. © 1994 Academic Press. All rights reserved.
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页码:216 / 223
页数:8
相关论文
共 36 条
[1]   CONTROL OF ALVEOLAR SURFACTANT IN RATS AT REST AND DURING PROLONGED HYPERPNOEA - PHARMACOLOGICAL EVIDENCE FOR 2 TISSUE POOLS OF SURFACTANT [J].
BARR, HA ;
NICHOLAS, TE ;
POWER, JHT .
BRITISH JOURNAL OF PHARMACOLOGY, 1988, 93 (03) :473-482
[2]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[3]   THE PERFUSED ISOLATED LUNG AS A POSSIBLE MODEL FOR THE STUDY OF LIPID-SYNTHESIS BY TYPE-II CELLS IN THEIR NATURAL-ENVIRONMENT [J].
BATENBURG, JJ ;
POST, M ;
OLDENBORG, V ;
VANGOLDE, LMG .
EXPERIMENTAL LUNG RESEARCH, 1980, 1 (01) :57-65
[4]   DEGRADATION OF VASOACTIVE INTESTINAL PEPTIDE BY ISOLATED, VENTILATED, AND PERFUSED RAT LUNGS [J].
BERNHARD, W ;
BOTHESANDTFORT, E ;
KOOP, H ;
CASSEL, W ;
VONWICHERT, P .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1989, 19 (06) :506-513
[5]  
Bils R F, 1980, Int Rev Exp Pathol, V21, P195
[6]  
BLANK ML, 1978, AM REV RESPIR DIS, V117, P273
[7]   PULMONARY NO2 TOXICITY - PHOSPHATIDYLCHOLINE LEVELS AND INCORPORATION OF [H-3]THYMIDINE INTO DNA [J].
BLANK, ML ;
DALBEY, W ;
CRESS, EA ;
GARFINKEL, S ;
SNYDER, F .
ENVIRONMENTAL RESEARCH, 1982, 27 (02) :352-360
[8]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[9]  
BROWN LAS, 1981, J BIOL CHEM, V256, P66
[10]   ROLE OF MICROTUBULES IN SURFACTANT SECRETION [J].
BROWN, LAS ;
PASQUALE, SM ;
LONGMORE, WJ .
JOURNAL OF APPLIED PHYSIOLOGY, 1985, 58 (06) :1866-1873