EFFECTIVENESS OF TALC AS ADSORBENT FOR PURIFICATION AND IMMOBILIZATION OF PLANT LIPOXYGENASES

被引：8

作者：

BATTU, S ^{[1
]}

RABINOVITCHCHABLE, H ^{[1
]}

BENEYTOUT, JL ^{[1
]}

机构：

[1] FAC MED LIMOGES, BIOCHIM LAB, CNRS, URA 1485, F-87025 LIMOGES, FRANCE

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1994年 / 42卷 / 10期

关键词：

PLANT LIPOXYGENASES; ENZYME ADSORPTION; TALC; PURIFICATION; STABILIZATION;

D O I：

10.1021/jf00046a008

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The present study describes the immobilization of lipoxygenase from Solanum tuberosum tuber and Lupinus albus, Cicer arietinum, and Pisum sativum seeds on a magnesium silicate based support, talc. In all cases, an increase in specific activity of many hundreds of units per milligram was obtained and the use of free or immobilized enzymes was quite similar. The adsorption of plant lipoxygenases on talc was a direct, rapid, and inexpensive method to purify and stabilize these enzymes.

引用

页码：2115 / 2119

页数：5

共 21 条

[1] SOYBEAN LIPOXYGENASE .1. PURIFICATION AND EFFECT OF ORGANIC SOLVENTS UPON KINETICS OF REACTION [J].

ALLEN, JC .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1968, 4 (02) :201-&

[2] OXODIENE FORMATION DURING THE VICIA-SATIVA LIPOXYGENASE-CATALYZED REACTION - OCCURRENCE OF DIOXYGENASE AND FATTY-ACID LYASE ACTIVITIES ASSOCIATED IN A SINGLE PROTEIN [J].

ANDRIANARISON, RH ;

RABINOVITCHCHABLE, H ;

BENEYTOUT, JL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) :1002-1009

[3] STABILIZATION OF POTATO-TUBER LIPOXYGENASE ON TALC [J].

BATTU, S ;

COOKMOREAU, J ;

BENEYTOUT, JL .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1994, 1211 (03) :270-276

[4] PROPERTIES OF A LIPOXYGENASE IN GREEN-ALGAE (OSCILLATORIA SP) [J].

BENEYTOUT, JL ;

ANDRIANARISON, RH ;

RAKOTOARISOA, Z ;

TIXIER, M .

PLANT PHYSIOLOGY, 1989, 91 (01) :367-372

[5]

BUCHHOLZ K, 1987, METHOD ENZYMOL, V135, P3

[6]

CHEBLI C, 1993, THESIS U P SABATIER

[7] RECENT INVESTIGATIONS INTO THE LIPOXYGENASE PATHWAY OF PLANTS [J].

GARDNER, HW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1084 (03) :221-239

[8] MODIFICATION OF COURSE OF REACTION BETWEEN WHEAT FLOUR LIPOXYGENASE AND LINOLEIC ACID DUE TO ADSORPTION OF LIPOXYGENASE ON GLUTENIN [J].

GRAVELAN.A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 41 (02) :427-&

[9] PRESENCE OF FATTY-ACID DEGRADING ENZYME IN A CERTAIN VARIETY OF PEAS (PISUM-SATIVUM-HORTENSE CV SOLARA) [J].

GUERDAM, E ;

ANDRIANARISON, RH ;

RABINOVITCHCHABLE, H ;

TIXIER, M ;

BENEYTOUT, JL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (10) :1593-1597

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

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