CYTOPLASMIC FREE-CA2+ LEVEL RISES WITH REPELLENTS AND FALLS WITH ATTRACTANTS IN ESCHERICHIA-COLI CHEMOTAXIS

被引：56

作者：

TISA, LS

ADLER, J

机构：

[1] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706

[2] UNIV WISCONSIN,DEPT GENET,MADISON,WI 53706

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 23期

关键词：

D O I：

10.1073/pnas.92.23.10777

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca2+-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at approximate to 100 nM when no stimuli were encountered, but changes in bacterial behavior correlated with changes in cytoplasmic free-Ca2+ concentration. For chemotactically wild-type E. coli, addition of a repellent resulted in cells tumbling and a transient increase in cytoplasmic free-Ca2+ levels. Conversely, addition of an attractant to wild-type cells caused running and produced a transient decrease in cytoplasmic free-Ca2+ levels. Studies with mutant strains showed that the chemoreceptors were required for the observed changes in cytoplasmic free-Ca2+ levels in response to chemical stimuli.

引用

页码：10777 / 10781

页数：5

共 37 条

[1] NONCHEMOTACTIC MUTANTS OF ESCHERICHIA COLI [J].