SIMPLE 2-STEP SEDIMENT EXTRACTION PROCEDURE FOR USE IN GENOTOXICITY AND TOXICITY BIOASSAYS

被引:37
作者
KWAN, KK [1 ]
DUTKA, BJ [1 ]
机构
[1] NATL WATER RES INST BRANCH,CANADA CTR INLAND WATERS,DEPT ENVIRONM,RIVERS RES BRANCH,BURLINGTON L7R 4A6,ONTARIO,CANADA
来源
TOXICITY ASSESSMENT | 1990年 / 5卷 / 04期
关键词
D O I
10.1002/tox.2540050407
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Three simple sediment extraction procedures, using Milli‐Q water, DMSO, and methanol, were applied to 16 sediment samples collected from the Thames River, Ontario, Canada. The three extracts were tested for the presence of toxicity and genotoxicity using the Microtox,® Toxi‐Chromotest,® and Mutatox® (with and without S9) procedures. Results of the study indicated that the Mutatox genotoxicity screening test was an extremely sensitive procedure responding to chemicals in all three types of extracts. Methanol was found to be more efficient than DMSO in extracting toxic and genotoxic chemicals from these sediments. Procedural details and results are discussed. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company
引用
收藏
页码:395 / 404
页数:10
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