STUDIES ON THE TURNOVER OF PROTEINS OF THE RAT ERYTHROCYTE-MEMBRANE

The membrane proteins of erythrocytes were labeled by injecting l-[14C]leucine and later l-[3H]leucine into rats, the two injections being 31 days apart. Control animals received the two isotopic forms of l-leucine simultaneously. Deviations in labeling ratio from control patterns were found on sodium dodecyl sulfate-polyacrylamide gel electrophorograms in restricted regions suggestive of turnover or loss of a few small proteins from the membrane between the 31 days. Most of the ghost proteins show no turnover. © 1979.