THE POLYMERASE CHAIN-REACTION FOR THE DETECTION OF HIV-1 GENOMIC RNA IN PLASMA FROM INFECTED INDIVIDUALS

被引:41
作者
OTTMANN, M
INNOCENTI, P
THENADEY, M
MICOUD, M
PELLOQUIN, F
SEIGNEURIN, JM
机构
[1] PASTEUR MERIEUX,LYONS,FRANCE
[2] CHU GRENOBLE,SERV MALAD INFECT,F-38043 GRENOBLE,FRANCE
关键词
HIV; PCR; PLASMA; VIREMIA;
D O I
10.1016/0166-0934(91)90165-V
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
HIV (human immunodeficiency virus) viraemia in serum or plasma of HIV-infected individuals was investigated by the polymerase chain reaction assay (PCR) in combination with reverse transcription to detect HIV-1 genomic RNA. Before PCR, plasma or serum was ultracentrifuged, precipitated virions were then treated with a RNase-free DNase, and a cDNA from the HIV-1 genomic RNA was synthesized. Thirty-three fresh plasma and seven sera from either HIV-1 antibody-positive individuals or patients treated with AZT were tested. Plasma from three patients were assayed 3 or 6 months apart. Twelve sera from HIV-1 antibody-negative individuals were used as negative control. PCR was performed with primers in LTR, gag and env regions: 11 of 40 samples were positive with three primer pairs, 16 with two primer pairs and 11 with only one primer pair. PCR on HIV-1 genomic cDNA was positive in 38 out of the 40 plasma or serum samples (95%), regardless of the clinical stage of the infection: HIV-1 was detected in 14 of the 15 untreated subjects and in 24 of the 25 AZT-treated patients. HIV p24 antigen was detected in the serum of 38% of subjects (15 of 40). The results suggest that this method is suitable for the detection of viral particles in plasma or serum from HIV-1-infected individuals irrespective of antiviral treatment.
引用
收藏
页码:273 / 284
页数:12
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