SEQUENCE AND EXPRESSION OF A RAT CDNA FOR LECAM-1

被引:31
作者
WATANABE, T
SONG, Y
HIRAYAMA, Y
TAMATANI, T
KUIDA, K
MIYASAKA, M
机构
[1] TOKYO METROPOLITAN INST MED SCI,DEPT IMMUNOL,TOKYO 113,JAPAN
[2] JAPANESE FDN CANC RES,INST CANC,DEPT VIRAL ONCOL,TOKYO 170,JAPAN
关键词
ADHESION MOLECULE; LECAM-1; CDNA; (RAT);
D O I
10.1016/0167-4781(92)90033-V
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A rat cDNA clone encoding an adhesion molecule, LECAM-1, has been isolated from the SD rat and the partial nucleotide sequence was determined. It encodes a peptide of 372 amino acids (aa), including a signal peptide of 38 aa. The protein has three tandem domains: a lectin domain, an EGF-like domain and two repeats of complement regulatory proteins (CR domain). The lectin binding domain has 93.2% and 81.4% and the EGF-like domain has 85.3% and 76.5% aa identity with those of mouse and human, respectively. In the CR repeat domain, the amino acid identity was 72.6% between human and rat and 71.8% between mouse and rat. Northern blot analysis detects the main transcript of about 3 kb in peripheral blood mononuclear cells (PBMC), spleen and thymus. The expression was down-regulated by mitogen stimulation of PBMC and spleen T cells. The protein encoded by this cDNA interacted with PPME when it was expressed on gp90MEL-14 negative mouse EL-4 cells.
引用
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页码:321 / 324
页数:4
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