GOLGI METHOD WITHOUT OSMIUM-TETROXIDE FOR THE STUDY OF THE CENTRAL-NERVOUS-SYSTEM

被引：61

作者：

GONZALEZBURGOS, I ^{[1
]}

TAPIAARIZMENDI, G ^{[1
]}

FERIAVELASCO, A ^{[1
]}

机构：

[1] MEXICAN INST SOCIAL SECUR,WESTERN BIOMED INVEST UNIV,DIV EXPTL PATHOL,GUADALAJARA 44420,JALISCO,MEXICO

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1992年 / 67卷 / 05期

关键词：

GOLGI STAIN; OSMIUM TETROXIDE; FORMALDEHYDE; CENTRAL NERVOUS SYSTEM;

D O I：

10.3109/10520299209110037

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A variant Golgi technique was developed that consisted of substituting osmium tetroxide with formaldehyde as the initial fixative in intracardiac perfusion, along with the addition of glacial acetic acid to the chromating fluid. This procedure avoids disposal of dangerous waste substances into the environment. Other advantages include 1) reduction of cost, danger to lab workers, and risk of disruption of the tissue slices during their handling by eliminating the osmium tetroxide, 2) clear tissue background, 3) greater quantity of impregnated neurons than in the classical procedure, with distinct morphological details easily identified even in gross sections and 4) reduction in processing time.

引用

页码：288 / 296

页数：9

共 21 条

[1]

CHROMATION AT LOW-TEMPERATURES IMPROVES IMPREGNATION OF NEURONS IN GOLGI-ALDEHYDE METHODS [J].