ISOLATION OF A POPLAR AND AN ARABIDOPSIS-THALIANA DIHYDRODIPICOLINATE SYNTHASE CDNA CLONE

被引：25

作者：

VAUTERIN, M

JACOBS, M

机构：

[1] Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, St.-Genesius Rode, B-1640

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 03期

关键词：

ARABIDOPSIS THALIANA; DIHYDRODIPICOLINATE SYNTHASE; FUNCTIONAL COMPLEMENTATION; POLYMERASE CHAIN REACTION; POPLAR;

D O I：

10.1007/BF00043882

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A poplar DHDPS cDNA clone has been isolated by functional rescue of the dapA-deficient AT997 mutant of Escherichia coli. By sequence comparison between the polar and maize DHDPS cDNAs, two oligonucleotides were designed to perform polymerase chain reaction (PCR) on Avabidopsis thaliana genomic DNA. The PCR fragment was subsequently used to isolate an Arabidopsis DHDPS genomic and cDNA clone.

引用

收藏

页码：545 / 550

页数：6

相关论文

共 20 条

[1] NUCLEOTIDE-SEQUENCE OF THE DAPA GENE FROM CORYNEBACTERIUM-GLUTAMICUM [J].

BONNASSIE, S ;

OREGLIA, J ;

SICARD, AM .

NUCLEIC ACIDS RESEARCH, 1990, 18 (21) :6421-6421

[2]

CHEN NY, 1993, J BIOL CHEM, V268, P9448

[3] NUCLEOTIDE-SEQUENCE OF A COMPLEMENTARY-DNA ENCODING O-METHYLTRANSFERASE FROM POPLAR [J].

DUMAS, B ;

VANDOORSSELAERE, J ;

GIELEN, J ;

LEGRAND, M ;

FRITIG, B ;

VANMONTAGU, M ;

INZE, D .

PLANT PHYSIOLOGY, 1992, 98 (02) :796-797

[4] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[5] 2 FEEDBACK-INSENSITIVE ENZYMES OF THE ASPARTATE PATHWAY IN NICOTIANA-SYLVESTRIS [J].

FRANKARD, V ;

GHISLAIN, M ;

JACOBS, M .

PLANT PHYSIOLOGY, 1992, 99 (04) :1285-1293

[6] DIRECT GENETIC SELECTION OF A MAIZE CDNA FOR DIHYDRODIPICOLINATE SYNTHASE IN AN ESCHERICHIA-COLI DAPA- AUXOTROPH [J].

FRISCH, DA ;

TOMMEY, AM ;

GENGENBACH, BG ;

SOMERS, DA .

MOLECULAR & GENERAL GENETICS, 1991, 228 (1-2) :287-293

[7] ISOLATION AND CHARACTERIZATION OF DIHYDRODIPICOLINATE SYNTHASE FROM MAIZE [J].

FRISCH, DA ;

GENGENBACH, BG ;

TOMMEY, AM ;

SELLNER, JM ;

SOMERS, DA ;

MYERS, DE .

PLANT PHYSIOLOGY, 1991, 96 (02) :444-452

[8] A CONSERVED CLEAVAGE-SITE MOTIF IN CHLOROPLAST TRANSIT PEPTIDES [J].

GAVEL, Y ;

VONHEIJNE, G .

FEBS LETTERS, 1990, 261 (02) :455-458

[9] DIHYDRODIPICOLINATE SYNTHASE OF NICOTIANA-SYLVESTRIS, A CHLOROPLAST-LOCALIZED ENZYME OF THE LYSINE PATHWAY [J].

GHISLAIN, M ;

FRANKARD, V ;

JACOBS, M .

PLANTA, 1990, 180 (04) :480-486

[10] PURIFICATION AND CHARACTERIZATION OF DIHYDRODIPICOLINATE SYNTHASE FROM WHEAT SUSPENSION-CULTURES [J].

KUMPAISAL, R ;

HASHIMOTO, T ;

YAMADA, Y .

PLANT PHYSIOLOGY, 1987, 85 (01) :145-151