SOLUBILIZATION OF CALFSKIN COLLAGEN IN CITRATE BUFFER WITH USE OF AUTOMATED EQUIPMENT

被引:5
作者
KAHN, LD
WITNAUER, LP
机构
[1] Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture, Philadelphia, Pennsylvania
关键词
D O I
10.1002/app.1969.070130115
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer. Copyright © 1969 John Wiley & Sons, Inc.
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页码:141 / &
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