IMMOBILIZATION OF CANE AMYLASE AND ACID-PHOSPHATASE ON TRICALCIUM PHOSPHATE (TCP) GEL

被引:17
作者
DAS, G
PRABHU, KA
机构
[1] Sugar Chemistry Department, National Sugar Institute, Kanpur, Kalyanpur
关键词
amylase; Immobilization; phosphatase; sugar cane; tricalcium phosphate;
D O I
10.1016/0141-0229(90)90138-G
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Amylase (E.C.3.2.1.1) and acid phosphatase (E.C.3.1.3.2) from sugar cane juice have been isolated and purified by salt and solvent fractionation. They have been separated by ion-exchange chromatography using DEAE cellulose and phosphocellulose columns and were immobilized on tricalcium phosphate gel. Some of the properties of immobilized and free soluble enzymes have been compared. The pH and temperature profiles of free and immobilized amylase and acid phosphatase did not show much alteration. Thermal stability of both the enzymes increased in the presence of 18% sucrose solution. The Km value of amylase and acid phosphatase increased on immobilization, while the Vmax values showed wide variation. Application of co-immobilized amylase and acid phosphatase in the removal of starch from cane juice has been suggested. © 1990.
引用
收藏
页码:625 / 630
页数:6
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