ALTERED POST-RECEPTORIAL SIGNAL TRANSDUCTION MECHANISM UNDER VARIOUS STIMULATION IN POLYMORPHONUCLEAR GRANULOCYTES OF ALZHEIMERS-DISEASE

The cyclic nucleotide changes were studied under 10-6 M isoproterenol (IP), 10-6 M carbachol and 10-8 M Met-enkephalin (Met-enk) stimulations in polymorphonuclear granulocytes (PMNLs) of middle-aged (aged 35-52 years) and elderly (aged 61-97 years) healthy subjects, as well as of patients with Alzheimer's disease (AD) (aged 58-65 years). From our results we can conclude that in the case of middle-aged healthy subjects only the IP caused a marked cAMP elevation while in elderly and AD all the applied substances stimulated the cAMP at different degrees. Concerning the cGMP levels in PMNLs, we observed a marked increase under carbachol and Met-enk stimulation, in middle-aged subjects, while in the elderly a weak change was obtained by carbachol. In AD practically no change of cGMP levels could be obtained. Thus, the main features of AD are a cAarP response to Met-enk and an abolition of a GarP response to carbachol. We can conclude that in PMNLs of elderly and patients with AD we assist to an altered post-receptorial signal transduction mechanism, which seems to be even more marked in the case of AD comparing to normal aging. © 1990.