NEUROTENSIN AND GROWTH HORMONE-RELEASING FACTOR-CONTAINING NEURONS PROJECTING TO THE MEDIAN-EMINENCE OF THE RAT - A COMBINED RETROGRADE TRACING AND IMMUNOHISTOCHEMICAL STUDY
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NIIMI, M
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KAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPANKAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPAN
NIIMI, M
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TAKAHARA, J
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KAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPANKAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPAN
TAKAHARA, J
[1
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SATO, M
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KAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPANKAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPAN
SATO, M
[1
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KAWANISHI, K
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KAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPANKAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPAN
KAWANISHI, K
[1
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机构:
[1] KAGAWA MED SCH,DEPT INTERNAL MED 1,KAGAWA,JAPAN
The origins of the neurotensin (NT)-containing nerve terminals in the median eminence were determined in rats by a combination of retrograde labeling with True blue and immunofluorescence staining. Moreover, the distribution of NT and growth hormone-releasing factor (GRF) was analyzed in the arcuate nucleus (ARC) with an elution-restaining procedure. The retrogradely labeled NT neurons were found mainly in the ARC. Only a few labeled NT neurons were seen in the periventricular and the paraventricular nuclei. The elution restaining procedure demonstrated that an average of 43% of the labeled NT cells in the ventral part of the ARC restained for GRF. These findings support the hypothesis of co-expression of NT with GRF from the ARC.