THE EXTRACELLULAR CYTOLYSIN OF VIBRIO-VULNIFICUS - INACTIVATION AND RELATIONSHIP TO VIRULENCE IN MICE

被引:103
作者
WRIGHT, AC
MORRIS, JG
机构
[1] UNIV MARYLAND,SCH MED,DEPT MED,DIV GEOG MED,BALTIMORE,MD 21201
[2] DEPT VET AFFAIRS MED CTR,BALTIMORE,MD 21218
关键词
D O I
10.1128/IAI.59.1.192-197.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The ca. 51-kDa extracellular cytolysin of Vibrio vulnificus has been proposed as a virulence factor. We inactivated the structural gene for cytolysin in fully virulent, clinical V. vulnificus strains by both transposon mutagenesis and marker exchange techniques. Inactivation of the cytolysin did not affect virulence in our mouse models. The 50% lethal dose of cytolysin-negative strains was comparable to that of the cytolysin-positive parent strains after intraperitoneal inoculation with and without iron loading. Similar results were obtained after intradermal injection: cytolysin-positive and -negative strains had the same 50% lethal dose and caused comparable tissue damage. While we cannot say that the cytolysin has no effect on the pathogenesis of V. vulnificus infections, its role appears to be of much less importance than are other factors, such as encapsulation.
引用
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页码:192 / 197
页数:6
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