ESCHERICHIA-COLI DIHYDRODIPICOLINATE SYNTHASE - CHARACTERIZATION OF THE IMINE INTERMEDIATE AND THE PRODUCT OF BROMOPYRUVATE TREATMENT BY ELECTROSPRAY MASS-SPECTROMETRY

被引：43

作者：

BORTHWICK, EB

CONNELL, SJ

TUDOR, DW

ROBINS, DJ

SHNEIER, A

ABELL, C

COGGINS, JR

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,LANARK,SCOTLAND

[2] UNIV GLASGOW,DEPT CHEM,GLASGOW G12 8QQ,LANARK,SCOTLAND

[3] UNIV CAMBRIDGE,CHEM LAB,DEPT CHEM,CAMBRIDGE CB2 1EW,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1995年 / 305卷

关键词：

D O I：

10.1042/bj3050521

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Simplified procedures for assaying and purifying dihydrodipicolinate synthase (EC 4.2.1.52), the first enzyme of the lysine biosynthetic pathway, have been developed and electrospray ionization m.s. has been used to observe the imine intermediate with pyruvate and to investigate the reaction of the enzyme with bromopyruvate and fluoropyruvate.

引用

页码：521 / 524

页数：4

共 18 条

[1]

BLACK S, 1954, J BIOL CHEM, V213, P50

[2]

BLACK S, 1954, J BIOL CHEM, V213, P39

[3] NUCLEOTIDE-SEQUENCE OF THE DAPA GENE FROM CORYNEBACTERIUM-GLUTAMICUM [J].