USE OF COMBINED SHEWHART-CUSUM CONTROL CHARTS IN INTERNAL QUALITY-CONTROL OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE TYPING OF FOOT-AND-MOUTH-DISEASE VIRUS-ANTIGEN

被引：13

作者：

BLACKSELL, SD

GLEESON, LJ

LUNT, RA

CHAMNANPOOD, C

机构：

[1] Commonwealth Scientific and Industrial Research Organisation, Australian Animal Health Laboratory, Geelong, Victoria

来源：

REVUE SCIENTIFIQUE ET TECHNIQUE DE L OFFICE INTERNATIONAL DES EPIZOOTIES | 1994年 / 13卷 / 03期

关键词：

ELISA; FOOT AND MOUTH DISEASE; QUALITY CONTROL; SHEWHART-CUSUM CONTROL CHARTS;

D O I：

10.20506/rst.13.3.785

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) for the typing of foot and mouth disease virus (FMDV) antigen was employed for the routine laboratory diagnosis of FMD at a regional veterinary laboratory in northern Thailand. An objective procedure was developed to monitor the test performance of the ELISA, using absolute test control limits in a Shewhart-CUSUM (cumulative sum) control chart method. The procedure detected significant data trends and 'beyond control limit' situations for each antigen typing system (types O, A and Asia 1), using an assay variable (gamma(i)). Retrospective analysis using Shewhart-CUSUM control charts of data from 42 ELISAs demonstrated that control limits were exceeded in two assays for FMDV type A. The Shewhart-CUSUM control chart is a simple and reliable internal quality control method for the detection of significant random and systematic variation in assays.

引用

页码：687 / 699

页数：13