IDENTIFICATION OF A PATIENT WITH STREPTOCOCCUS-PNEUMONIAE BACTEREMIA AND MENINGITIS BY THE POLYMERASE CHAIN-REACTION (PCR)

被引：18

作者：

ISAACMAN, DJ

ZHANG, YZ

RYDQUISTWHITE, J

WADOWSKY, RM

POST, JC

EHRLICH, GD

机构：

[1] UNIV PITTSBURGH,SCH MED,DEPT PATHOL,PITTSBURGH,PA

[2] UNIV PITTSBURGH,SCH MED,DEPT PEDIAT,PITTSBURGH,PA 15261

[3] UNIV PITTSBURGH,SCH MED,DEPT INFECT DIS & MICROBIOL,PITTSBURGH,PA

[4] UNIV PITTSBURGH,SCH MED,DEPT OTOLARYNGOL,PITTSBURGH,PA

[5] GRAD SCH PUBL HLTH,PITTSBURGH,PA

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 03期

关键词：

POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE; BACTEREMIA; CHILDREN;

D O I：

10.1006/mcpr.1995.0026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction (PCR) assay based on the penicillin-binding protein gene PBP2B identified the presence of DNA specific for Streptococcus pneumoniae in the serum and CSF of a patient with culture-proven bacteremia and meningitis. Positive signals were seen to dilutions of 1:125 and 1:390,625 for the blood and CSF specimens, respectively. Potential advantages of PCR over conventional culture include exquisite sensitivity, faster results and the ability to identify the organisms by the presence of species-specific DNA even in patients pretreated with antibiotics.

引用

页码：157 / 160

页数：4

共 24 条

[1]

Dowson C.G., Hutchison A., Woodford N., Johnson A.P., George R.C., Spratt B.G., Penicillin-resistant Viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae, Proceedings of the National Academy of Science USA, 87, pp. 5858-5862, (1990)

[2]

Ehrlich G.D., PCR-based laboratory methods for the detection of the human retroviridae and hepad-naviridae, Pcr-Based Diagnostics for Infectious Disease, pp. 4l5-416, (1993)

[3]

Ehrlich G.D., Greenberg S., Abbott M., Detection of human T-cell lymphoma/leukemia viruses (HTLV), PCR Protocols: A Guide to Methods and Applications, pp. 325-336, (1990)

[4]

Brisson-Noel A., Gicquel B., Lecossier D., Levy-Frebault V., Nassif X., Hance A.J., Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples, Lancet, 5, pp. 1069-1071, (1989)

[5]

Burstain J.M., Grimprel E., Lukehart S.A., Norgard M.V., Radolf J.D., Sensitive detection of Treponema pallidum by using the polymerase chain reaction, Journal of Clinical Microbiology, 29, pp. 62-69, (1991)

[6]

Demmier G.J., Buffone G.J., Schimbor C.M., May R.A., Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification, Journal of Infectious Diseases, 158, pp. 1177-1184, (1988)

[7]

Isaacman D.J., Zhang Y., Wald E.R., Wadowsky R., Ehrlich G., A sensitive and specific assay utilizing the polymerase chain reaction for the detection of 5. Pneumoniae in blood, Pediatric Research, 35, (1994)

[8]

Kwok S., Mack D.H., Mullis K.B., Poiesz B., Ehrlich G., Blair D., Friedman-Kien A., Sninsky J.J., Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection, Journal of Virology, 61, pp. 1690-1694, (1987)

[9]

Morris B.J., Flanagan J.L., McKinnon K.J., Nightingale B.N., Papillomavirus screening of cervical lavages by polymerase chain reaction, Lancet, 5, (1988)

[10]

Shankar P., Manjunath N., Lakshmi R., Aditi B., Seth P., Shriniwas S., Identification of Mycobacterium tuberculosis by polymerase chain reaction, Lancet, 335, (1990)

← 1 2 3 →