HUMAN ARYLHYDROCARBON RECEPTOR - FUNCTIONAL EXPRESSION AND CHROMOSOMAL ASSIGNMENT TO 7P21

被引：33

作者：

EMA, M

MATSUSHITA, N

SOGAWA, K

ARIYAMA, T

INAZAWA, J

NEMOTO, T

OTA, M

OSHIMURA, M

FUJIIKURIYAMA, Y

机构：

[1] TOHOKU UNIV,FAC SCI,DEPT CHEM,SENDAI,MIYAGI 980,JAPAN

[2] KYOTO PREFECTURAL UNIV MED,DEPT HYG,KAMIGYO KU,KYOTO 602,JAPAN

[3] IWATE MED UNIV,SCH DENT,DEPT BIOCHEM,MORIOKA,IWATE 020,JAPAN

[4] NAGOYA UNIV,DEPT AGR CHEM,CHIKUSA KU,NAGOYA,AICHI 46401,JAPAN

[5] TOTTORI UNIV,SCH LIFE SCI,YONAGO,TOTTORI 683,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 116卷 / 04期

关键词：

AH RECEPTOR; DIOXIN BINDING ACTIVITY; CHROMOSOMAL LOCALIZATION; XRE BINDING ACTIVITY;

D O I：

10.1093/oxfordjournals.jbchem.a124605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. The overall amino acid identity with mouse AhR from cell line Hepa-1 is 72.5%. The human AhR expressed either in COS-7 cells or in a reticulocyte lysate in vitro translation system showed specific dioxin-binding activity and Arnt-dependent DNA-binding activity. Chromosomal localization of the AhR gene was determined to be chromosome 7p21 by fluorescent in situ hybridization and DNA blot hybridization using 23 human X mouse or Chinese hamster hybrid cell DNAs.

引用

页码：845 / 851

页数：7

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