USE OF POLYSTYRENE GELS IN PREPARATIVE RECYCLE-HPLC FOR THE SEPARATION OF STRUCTURALLY RELATED MOLECULES

被引:13
作者
LESEC, J [1 ]
QUIVORON, C [1 ]
机构
[1] ECOLE SUPER PHYS & CHIM IND, F-75231 PARIS 05, FRANCE
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 04期
关键词
D O I
10.1080/01483917908060077
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper deals with the use of polystyrene gels in high performance liquid chromatography for preparative separations of small molecules with very similar structures. The preparative chromatograph, equipped with a recycle device and made in our laboratory, is described. The column set, consisted of three columns packed with low porosity polystyrene gel. The mobile phase was diisopropylether. Three particle sizes (10u, 20u, 50u) were studied for improving the preparative performance. We checked the very high efficiency of our recycling system versus sample load. High sample loadings(up to 100 grams) can be injected when the separation does not require a large number of plates but typical sample loads are 10–20 grams for high performance separations. Some examples of separation of diastereoisomers, configurational isomers or related structure molecules are given. The advantages of our preparative system are discussed. © 1979, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码:467 / 484
页数:18
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