SENSITIVE METHOD FOR HPLC DETERMINATION OF ACETYLCHOLINE, CHOLINE AND THEIR ANALOGS USING FLUOROMETRIC DETECTION

被引：15

作者：

RICNY, J

TUCEK, S

VINS, I

机构：

[1] CZECHOSLOVAK ACAD SCI, INST PHYSIOL, VIDENSKA 1083, CS-14220 PRAGUE, CZECHOSLOVAKIA

[2] TESSEK LTD, CS-11011 PRAGUE, CZECHOSLOVAKIA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1992年 / 41卷 / 01期

关键词：

ACETYLCHOLINE; FLUOROMETRIC DETERMINATION; CHOLINE; HPLC;

D O I：

10.1016/0165-0270(92)90119-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for reversed-phase HPLC separation of acetylcholine and choline and of their homologues in tissue extracts or perfusion fluids, combined with postcolumn enzymatic derivatization and fluorometric quantification. The separation occurs on a polymeric resin derivatized with hydrophobic moiety and the mobile phase consists of Na2HPO4, 3-(p-hydroxyphenyl)propionic acid and sodium dodecylsulphate; postcolumn enzyme reactor contains immobilized acetylcholinesterase, choline oxidase, and peroxidase. The limits of detection are 1 pmol choline and 3 pmol acetylcholine per sample. The method is free of interferences encountered with electrochemical detection and well suited for non-attended automatic operation.

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页码：11 / 17

页数：7

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