ELISA FOR QUANTITATION OF L-SELECTIN SHED FROM LEUKOCYTES INVIVO

被引:116
作者
SPERTINI, O
SCHLEIFFENBAUM, B
WHITEOWEN, C
RUIZ, P
TEDDER, TF
机构
[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV TUMOR IMMUNOL,44 BINNEY ST,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115
[3] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115
[4] UNIV CINCINNATI,MED CTR,SHRINERS BURN INST,CINCINNATI,OH 45267
[5] UNIV MIAMI,SCH MED,DEPT PATHOL,MIAMI,FL 33101
关键词
L-SELECTIN; SHEDDING; RECEPTOR; ELISA; LEUKOCYTE;
D O I
10.1016/0022-1759(92)90017-N
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
L-selectin is a cell surface receptor on granulocytes, lymphocytes and monocytes that is responsible for the initial attachment of leukocytes to endothelium. The extracellular domain Of L-selectin is proteolytically shed from leukocytes following cellular activation in vitro. The shed form of L-selectin (SL-selectin) is functionally active and at high concentrations can inhibit leukocyte attachment to endothelium. Therefore, an ELISA was developed to quantitate the levels Of SL-Selectin in biological fluids, biopsy specimens and during recombinant protein production. This simple, quantitative sandwich ELISA uses two monoclonal antibodies directed against the extracellular domain of SL-selectin. The assay has a detection range of 5-1300 ng/ml, is precise and sensitive. The ability of this assay to detect SL-selectin in serum, plasma, and culture supernatant fluid was demonstrated and it was used to quantitate circulating SL-selectin in normal and patient sera. Patients with sepsis and HIV infection showed markedly elevated SL-selectin levels in serum. Thus, the ELISA should prove useful both for laboratory purposes as well as in the diagnostic evaluation of patients with inflammatory diseases.
引用
收藏
页码:115 / 123
页数:9
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