CHARACTERIZATION AND SUBCELLULAR-LOCALIZATION OF TOMATO RINGSPOT NEPOVIRUS PUTATIVE MOVEMENT PROTEIN

被引:76
作者
WIECZOREK, A [1 ]
SANFACON, H [1 ]
机构
[1] AGR CANADA,RES STN,6660 NW MARINE DR,VANCOUVER V6T 1X2,BC,CANADA
关键词
D O I
10.1006/viro.1993.1314
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sequence comparison of the tomato ringspot nepovirus (TomRSV) genome with related viruses suggested that the region of the RNA-2-encoded polyprotein immediately upstream of the coat protein may be involved in the cell-to-cell movement of the virus (Rott et al., 1991, J. Gen. Virol. 72, 1505-1514). To further study the role of this portion of the genome, monoclonal antibodies against the putative movement protein were raised. Western blots of plant extracts allowed the detection of a viral nonstructural protein of M(r) 45K present only in TomRSV-infected tissues. Immunogold-labeling studies revealed that in Nicotiana clevelandii the putative movement protein was found only in infected cells immediately adjacent to the necrotic tissue, and that it was associated with tubular structures containing virus-like particles present in or near the cell wall. This provides further evidence that this protein is involved in the cell-to-cell movement of the virus and that this movement might take place via the formation of tubular structures. © 1993 American Health Foundation and Academic Press.
引用
收藏
页码:734 / 742
页数:9
相关论文
共 36 条
[1]  
Atabekov J G, 1990, Adv Virus Res, V38, P201, DOI 10.1016/S0065-3527(08)60863-5
[2]   THE NUCLEOTIDE-SEQUENCE OF RNA-2 OF RASPBERRY RINGSPOT NEPOVIRUS [J].
BLOK, VC ;
WARDELL, J ;
JOLLY, CA ;
MANOUKIAN, A ;
ROBINSON, DJ ;
EDWARDS, ML ;
MAYO, MA .
JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2189-2194
[3]   CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES [J].
CLARK, MF ;
ADAMS, AN .
JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) :475-483
[4]   INVITRO PROCESSING OF THE RNA-2-ENCODED POLYPROTEIN OF 2 NEPOVIRUSES - TOMATO BLACK RING VIRUS AND GRAPEVINE CHROME MOSAIC-VIRUS [J].
DEMANGEAT, G ;
HEMMER, O ;
FRITSCH, C ;
LE GALL, O ;
CANDRESSE, T .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :247-252
[5]   VIRUS-SPECIFIC PROTEINS IN CELLS INFECTED WITH TOMATO BLACK RING NEPOVIRUS - EVIDENCE FOR PROTEOLYTIC PROCESSING INVIVO [J].
DEMANGEAT, G ;
HEMMER, O ;
REINBOLT, J ;
MAYO, MA ;
FRITSCH, C .
JOURNAL OF GENERAL VIROLOGY, 1992, 73 :1609-1614
[6]  
DIFRANCO A, 1983, J SUBMICR CYTOL PATH, V15, P539
[7]  
GOLDBACH R, 1987, MICROBIOL SCI, V4, P197
[8]   THE AU-RICH SEQUENCES PRESENT IN THE INTRONS OF PLANT NUCLEAR PRE-MESSENGER RNAS ARE REQUIRED FOR SPLICING [J].
GOODALL, GJ ;
FILIPOWICZ, W .
CELL, 1989, 58 (03) :473-483
[9]  
GUAN C, 1988, GENE, V67, P21
[10]  
Harlow E, 1988, ANTIBODIES LAB MANUA