IXR1, A YEAST PROTEIN THAT BINDS TO PLATINATED DNA AND CONFERS SENSITIVITY TO CISPLATIN

被引：182

作者：

BROWN, SJ ^{[1
]}

KELLETT, PJ ^{[1
]}

LIPPARD, SJ ^{[1
]}

机构：

[1] MIT, DEPT CHEM, CAMBRIDGE, MA 02139 USA

来源：

SCIENCE | 1993年 / 261卷 / 5121期

关键词：

D O I：

10.1126/science.8342024

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Structure-specific recognition proteins (SSRPs) bind to DNA containing intrastrand cross-links formed by the anticancer drug cisplatin. A yeast gene encoding an SSRP, designated IXR1, was cloned and sequenced. The Ixr1 protein, a member of the high mobility group-box protein family, bound specifically to DNA modified with cisplatin but not inactive platinum compounds. A yeast strain with an inactivated IXR1 gene was half as sensitive to cisplatin and accumulated one-third as many platinum-DNA lesions after treatment with cisplatin as the parental strain. These findings suggest that SSRPs play a role in mediating the cytotoxicity of cisplatin.

引用

页码：603 / 605

页数：3

共 22 条

[1]

BROWN SDM, UNPUB

[2] ISOLATION AND CHARACTERIZATION OF HUMAN CDNA CLONES ENCODING A HIGH MOBILITY GROUP BOX PROTEIN THAT RECOGNIZES STRUCTURAL DISTORTIONS TO DNA CAUSED BY BINDING OF THE ANTICANCER AGENT CISPLATIN [J].