PERFUSING PIPETTES

被引：56

作者：

TANG, JM

WANG, J

QUANDT, FN

EISENBERG, RS

机构：

[1] RUSH MED COLL,DEPT PHYSIOL,1750 W HARRISON ST,CHICAGO,IL 60612

[2] RUSH MED COLL,CTR MULTIPLE SCLEROSIS,CHICAGO,IL 60612

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1990年 / 416卷 / 03期

关键词：

Patch clamp; Pipette perfusion; Solution exchange;

D O I：

10.1007/BF00392072

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

A simple modification of a patch-clamp set-up allows the fluid in a patch pipette to be changed. The time course of the solution exchange is estimated from the time course of change of the reversal potential of the current through an open Ca2+ activated K+channel: solution exchange takes less than 1 min. Measurements of the power spectrum of noise show that the modified set-up introduces little excess noise below 1 kHz. © 1990 Springer-Verlag.

引用

页码：347 / 350

页数：4

共 13 条

[1] POTASSIUM TRANSLOCATION BY THE NA+/K+ PUMP IS VOLTAGE INSENSITIVE
BAHINSKI, A
NAKAO, M
GADSBY, DC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) : 3412 - 3416
[2] SINGLE GLUTAMATE-ACTIVATED CHANNELS RECORDED FROM LOCUST MUSCLE-FIBERS WITH PERFUSED PATCH-CLAMP ELECTRODES
CULLCANDY, SG
MILEDI, R
PARKER, I
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1981, 321 (DEC): : 195 - 210
[3] VOLTAGE DEPENDENCE OF NA/K PUMP CURRENT IN ISOLATED HEART-CELLS
GADSBY, DC
KIMURA, J
NOMA, A
[J]. NATURE, 1985, 315 (6014) : 63 - 65
[4] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES
HAMILL, OP
MARTY, A
NEHER, E
SAKMANN, B
SIGWORTH, FJ
[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02): : 85 - 100
[5] Hille B, 1984, IONIC CHANNELS EXCIT
[6] HILLE E, 1964, ANALYSIS
[7] A NOVEL HOLDER ALLOWING INTERNAL PERFUSION OF PATCH-CLAMP PIPETTES
LAPOINTE, JY
SZABO, G
[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1987, 410 (1-2): : 212 - 216
[8] NEHER E, 1988, CALCIUM ION CHANNEL
[9] ISOLATION AND KINETIC-ANALYSIS OF INWARD CURRENTS IN NEURO-BLASTOMA CELLS
QUANDT, FN
NARAHASHI, T
[J]. NEUROSCIENCE, 1984, 13 (01) : 249 - 262
[10] 3 KINETICALLY DISTINCT POTASSIUM CHANNELS IN MOUSE NEURO-BLASTOMA CELLS
QUANDT, FN
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1988, 395 : 401 - 418

← 1 2 →