STUDIES ON THE MODE OF ACTION OF CALCIFEROL .18. EVIDENCE FOR A SPECIFIC HIGH-AFFINITY BINDING-PROTEIN FOR 1,25 DIHYDROXYVITAMIN-D3 IN CHICK KIDNEY AND PANCREAS

被引：109

作者：

CHRISTAKOS, S ^{[1
]}

NORMAN, AW ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)90942-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosol fractions prepared from rachitic chick kidney and pancreas were analyzed for binding of vitamin D3 metabolites by sucrose density gradient centrifugation. Both cytosol fractions were found to contain a 3.6S macromolecule which specifically binds 1,25-dihydroxy[3H] vitamin D3 and in addition a 5 to 6S macromolecule which binds 25-hydroxy[3H]vitamin D3. Sucrose gradient analysis of a KCl extract prepared from kidney or pancreas chromatin resulted in a peak (3.6S) of bound 1,25-dihydroxyvitamin D3 which could not be distinguished from the cytoplasmic binding component. The interaction of 1,25-dihydroxy[3H]vitamin D3 with the cytoplasmic binding component of both tissues occurred at low concentrations of hormone with high affinity. © 1979.

引用

页码：56 / 63

页数：8

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