ANALYSIS AND ISOLATION OF INDOLE ALKALOIDS OF FUNGI BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

An efficient analytical and isolation method was elaborated for biologically active tryptamines using a computer-aided liquid chromatographic-gas chromatographic system. The separation method includes a new efficient extraction procedure, optimization programme for high-performance liquid chromatographic separation, identification by diode-array detection and a spectrometric and electrochemical assay. The identification of indole alkaloids was confirmed by thin-layer and gas chromatography and mass spectrometry. The method was used for analysis and isolation of psychotropic substances in extracts from the fruit bodies of hallucinogenic fungi of genera Psilocybe, Inocybe and Amanita and in mycelial extracts from the species Psilocybe bohemica.